Author: ["Elibio L. Rech","Sergio J. Ochatt","Pradeep K. Chand","Michael R. Davey","Bernard J. Mulligan","John B. Power"]
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Abstract
DNA synthesis increased significantly in isolated Prunus and Solanumprotoplasts electroporated at 250V cm−1 for 87 μsec and 750V cm−1 for 29 μsec respectively. This increase correlates with an earlier onset of division of protoplast-derived cells in culture. Inhibition of cell wall synthesis did not affect electro-enhancement of DNA synthesis. Electrostimulation of DNA synthesis coupled with enhanced division may have applications to protoplast systems and plant genetic engineering in general.Cite this article
Rech, E., Ochatt, S., Chand, P. et al. Electroporation Increases DNA Synthesis in Cultured Plant Protoplasts. Nat Biotechnol 6, 1091–1093 (1988). https://doi.org/10.1038/nbt0988-1091 