Author: ["Tineke Rutgers","Daniel Gordon","Anne Marie Gathoye","Michael Hollingdale","Wayne Hockmeyer","Martin Rosenberg","Michel De Wilde"]
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Abstract
The immune dominant repetitive epitope of the circumsporozoite protein of Plasmodium falciparum was fused to the pre-S2 region of the surface antigen of hepatitis B virus within an expression cassette. Yeast cells transformed with vectors carrying the fusions synthesized hybrid proteins that assembled into particles similar to those formed in human serum from the surface antigen alone. These hybrid protein particles expose the circumsporozoite epitope on their exterior and induce a high titer antibody response towards the circumsporozoite epitope in immunized animals. The antibodies show typical circumsporozoite precipitin reactions and inhibit invasion of hepatoma cells in vitro, both indicative of a protective immune response. This formulation could prove useful as a candidate sporozoite malaria vaccine for human clinical trials.Cite this article
Rutgers, T., Gordon, D., Gathoye, A. et al. Hepatitis B Surface Antigen as Carrier Matrix for the Repetitive Epitope of the Circumsporozoite Protein of Plasmodium Falciparum.. Nat Biotechnol 6, 1065–1070 (1988). https://doi.org/10.1038/nbt0988-1065 