Author: ["Dennis E. McCabe","William F. Swain","Brian J. Martinell","Paul Christou"]
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Abstract
We have used particle acceleration by electric discharge to introduce DNA-coated gold particles into meristems of immature soybean seeds. Approximately 2 percent of shoots derived from these meristems via organogenesis were chimeric for expression of the introduced gene. Experiments using this protocol have resulted in mature RO and R1 plants expressing the introduced gene(s). We regard this work as a major step in the practical application of genetic engineering to the useful modification of food-crop species.Cite this article
McCabe, D., Swain, W., Martinell, B. et al. Stable Transformation of Soybean (Glycine Max) by Particle Acceleration. Nat Biotechnol 6, 923–926 (1988). https://doi.org/10.1038/nbt0888-923 