Author: ["Sundeep Kalantry","Kyle C. Mills","Della Yee","Arie P. Otte","Barbara Panning","Terry Magnuson"]
CITE.CC academic search helps you expand the influence of your papers.
Abstract
The Polycomb group (PcG) encodes an evolutionarily conserved set of chromatin-modifying proteins that are thought to maintain cellular transcriptional memory by stably silencing gene expression1. In mouse embryos that are mutated for the PcG protein Eed, X-chromosome inactivation (XCI) is not stably maintained in extra-embryonic tissues2. Eed is a component of a histone-methyltransferase complex that is thought to contribute to stable silencing in undifferentiated cells due to its enrichment on the inactive X-chromosome in cells of the early mouse embryo and in stem cells of the extra-embryonic trophectoderm lineage3,4,5,6,7,8. Here, we demonstrate that the inactive X-chromosome in Eed−/− trophoblast stem cells and in cells of the trophectoderm-derived extra-embryonic ectoderm in Eed−/− embryos remain transcriptionally silent, despite lacking the PcG-mediated histone modifications that normally characterize the facultative heterochromatin of the inactive X-chromosome. Whereas undifferentiated Eed−/− trophoblast stem cells maintained XCI, reactivation of the inactive X-chromosome occurred when these cells were differentiated. These results indicate that PcG complexes are not necessary to maintain transcriptional silencing of the inactive X-chromosome in undifferentiated stem cells. Instead, PcG proteins seem to propagate cellular memory by preventing transcriptional activation of facultative heterochromatin during differentiation.
Cite this article
Kalantry, S., Mills, K., Yee, D. et al. The Polycomb group protein Eed protects the inactive X-chromosome from differentiation-induced reactivation. Nat Cell Biol 8, 195–202 (2006). https://doi.org/10.1038/ncb1351