Author: ["Hidemasa Goto","Tohru Kiyono","Yasuko Tomono","Aie Kawajiri","Takeshi Urano","Koichi Furukawa","Erich A. Nigg","Masaki Inagaki"]
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Abstract
Mitotic chromosomal dynamics is regulated by the coordinated activities of many mitotic kinases1, such as cyclin-dependent kinase 1 (Cdk1)2,3, Aurora-B4 or Polo-like kinase 1 (Plk1)5, but the mechanisms of their coordination remain unknown. Here, we report that Cdk1 phosphorylates Thr 59 and Thr 388 on inner centromere protein (INCENP), which regulates the localization4 and kinase activity6,7,8 of Aurora-B from prophase to metaphase. INCENP depletion disrupts Plk1 localization specifically at the kinetochore. This phenotype is rescued by the exogenous expression of INCENP wild type and INCENP mutated at Thr 59 to Ala (T59A), but not at Thr 388 to Ala (T388A). The replacement of endogenous INCENP with T388A resulted in the delay of progression from metaphase to anaphase. We propose that INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore, and that the complex formation of Plk1 and Aurora-B on INCENP may play crucial roles in the regulation of chromosomal dynamics.Cite this article
Goto, H., Kiyono, T., Tomono, Y. et al. Complex formation of Plk1 and INCENP required for metaphase–anaphase transition. Nat Cell Biol 8, 180–187 (2006). https://doi.org/10.1038/ncb1350 