GCP-WD is a γ-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule n

Abstract

The γ-tubulin ring complex (γTuRC) is a large multi-protein complex that is required for microtubule nucleation from the centrosome. Here, we show that the GCP-WD protein (originally named NEDD1) is the orthologue of the Drosophila Dgrip71WD protein, and is a subunit of the human γTuRC. GCP-WD has the properties of an attachment factor for the γTuRC: depletion or inhibition of GCP-WD results in loss of the γTuRC from the centrosome, abolishing centrosomal microtubule nucleation, although the γTuRC is intact and able to bind to microtubules. GCP-WD depletion also blocks mitotic chromatin-mediated microtubule nucleation, resulting in failure of spindle assembly. Mitotic phosphorylation of GCP-WD is required for association of γ-tubulin with the spindle, separately from association with the centrosome. Our results indicate that GCP-WD broadly mediates targeting of the γTuRC to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation.

Cite this article

Lüders, J., Patel, U. & Stearns, T. GCP-WD is a γ-tubulin targeting factor required for centrosomal and chromatin-mediated microtubule nucleation. Nat Cell Biol 8, 137–147 (2006). https://doi.org/10.1038/ncb1349

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