Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell ster
Author: ["Inna A. Belyantseva","Erich T. Boger","Sadaf Naz","Gregory I. Frolenkov","James R. Sellers","Zubair M. Ahmed","Andrew J. Griffith","Thomas B. Friedman"]
Publication: Nature Cell Biology
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Abstract
Stereocilia are microvilli-derived mechanosensory organelles that are arranged in rows of graded heights on the apical surface of inner-ear hair cells1. The 'staircase'-like architecture of stereocilia bundles is necessary to detect sound and head movement, and is achieved through differential elongation of the actin core of each stereocilium to a predetermined length2,3. Abnormally short stereocilia bundles that have a diminished staircase are characteristic of the shaker 2 (Myo15ash2) and whirler (Whrnwi) strains of deaf mice4,5,6. We show that myosin-XVa is a motor protein that, in vivo, interacts with the third PDZ domain of whirlin through its carboxy-terminal PDZ-ligand. Myosin-XVa then delivers whirlin to the tips of stereocilia. Moreover, if green fluorescent protein (GFP)-Myo15a is transfected into hair cells of Myo15ash2 mice, the wild-type pattern of hair bundles is restored by recruitment of endogenous whirlin to the tips of stereocilia. The interaction of myosin-XVa and whirlin is therefore a key event in hair-bundle morphogenesis.
Cite this article
Belyantseva, I., Boger, E., Naz, S. et al. Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia. Nat Cell Biol 7, 148–156 (2005). https://doi.org/10.1038/ncb1219
