Author: ["Wilma A. Hofmann","Ljuba Stojiljkovic","Beata Fuchsova","Gabriela M. Vargas","Evangelos Mavrommatis","Vlada Philimonenko","Katarina Kysela","James A. Goodrich","James L. Lessard","Thomas J. Hope","Pavel Hozak","Primal de Lanerolle"]
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Abstract
Actin is abundant in the nucleus and has been implicated in transcription; however, the nature of this involvement has not been established. Here we demonstrate that β-actin is critically involved in transcription because antibodies directed against β-actin, but not muscle actin, inhibited transcription in vivo and in vitro. Chromatin immunoprecipitation assays demonstrated the recruitment of actin to the promoter region of the interferon-γ-inducible MHC2TA gene as well as the interferon-α-inducible G1P3 gene. Further investigation revealed that actin and RNA polymerase II co-localize in vivo and also co-purify. We employed an in vitro system with purified nuclear components to demonstrate that antibodies to β-actin block the initiation of transcription. This assay also demonstrates that β-actin stimulates transcription by RNA polymerase II. Finally, DNA-binding experiments established the presence of β-actin in pre-initiation complexes and also showed that the depletion of actin prevented the formation of pre-initiation complexes. Together, these data suggest a fundamental role for actin in the initiation of transcription by RNA polymerase II.Cite this article
Hofmann, W., Stojiljkovic, L., Fuchsova, B. et al. Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II. Nat Cell Biol 6, 1094–1101 (2004). https://doi.org/10.1038/ncb1182 