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Abstract
Identification of pathogen-specific T cells has been greatly facilitated by the advent of synthetic peptide–major histocompatibility complex (MHC) tetramers. In many cases, however, specific epitopes have not been defined, necessitating detection methods that function independently of exact peptide-MHC specificity. Lymphocytes acquire surface proteins from antigen-presenting cells (APCs), and we have exploited this phenomenon to develop the T-cell recognition of APCs by protein transfer (TRAP) assay. This method is based on biotinylation and streptavidin-fluorochrome labeling of APCs, followed by subsequent acquisition of this label by antigen-specific T cells. The TRAP procedure detects MHC class I–restricted T cells regardless of their cytokine profiles or peptide-MHC affinities, and provides a versatile tool for monitoring the phenomenon of APC membrane acquisition by antigen-specific T cells.Cite this article
Beadling, C., Slifka, M. Quantifying viable virus-specific T cells without a priori knowledge of fine epitope specificity. Nat Med 12, 1208–1212 (2006). https://doi.org/10.1038/nm1413 