Direct access to CD4+ T cells specific for defined antigens according to CD154 expression
Author: ["Marco Frentsch","Olga Arbach","Dennis Kirchhoff","Beate Moewes","Margitta Worm","Martin Rothe","Alexander Scheffold","Andreas Thiel"]
Publication: Nature Medicine
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Abstract
The direct assessment of T helper (TH)-cell responses specific for antigens is essential to evaluate pathogenic and protective immunity. Presently, analysis and isolation of antigen-specific TH cells is restricted to cells that produce cytokines, or can be performed only with a rare selection of specific peptide major histocompatibility complex class II (MHC II) multimers. Here we report a new method that enables the assessment and isolation of TH cells specific for a defined antigen according to CD154 expression induced after stimulation in vitro. We show that antigen-induced CD154 expression is highly sensitive and specific for human and mouse antigen-specific TH cells. Moreover, the isolation of antigen-specific CD154+ TH cells necessitates only surface staining with antibodies, thereby enabling the fast generation of antigen-specific TH cell lines. Our approach allows assessment of TH cells with a defined specificity for the combined quantitative and qualitative analysis of TH-cell immunity as well as for the isolation of specific TH cells for targeted cellular immunotherapies.
Cite this article
Frentsch, M., Arbach, O., Kirchhoff, D. et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat Med 11, 1118–1124 (2005). https://doi.org/10.1038/nm1292
