Identification of a new human coronavirus
Author: ["Lia van der Hoek","Krzysztof Pyrc","Maarten F Jebbink","Wilma Vermeulen-Oost","Ron J M Berkhout","Katja C Wolthers","Pauline M E Wertheim-van Dillen","Jos Kaandorp","Joke Spaargaren","Ben Berkhout"]
Publication: Nature Medicine
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Abstract
Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human population.
Cite this article
van der Hoek, L., Pyrc, K., Jebbink, M. et al. Identification of a new human coronavirus. Nat Med 10, 368–373 (2004). https://doi.org/10.1038/nm1024
