Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation
Author: ["Edward Brown","Trevor McKee","Emmanuelle diTomaso","Alain Pluen","Brian Seed","Yves Boucher","Rakesh K. Jain"]
Publication: Nature Medicine
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Abstract
The content and structure of collagen is essential in governing the delivery of therapeutic molecules in tumors. Thus, simple histological staining of tumor tissue biopsies for collagen could be used to assess the accessibility of molecular therapeutics in tumors. Here we show that it is possible to optically image fibrillar collagen in tumors growing in mice using second-harmonic generation (SHG). Using this noninvasive technique, we estimated relative diffusive hindrance, quantified the dynamics of collagen modification after pharmacologic intervention and provided mechanistic insight into improved diffusive transport induced by the hormone relaxin. This technology could offer basic scientists and clinicians an enhanced ability to estimate the relative penetrabilities of molecular therapeutics.
Cite this article
Brown, E., McKee, T., diTomaso, E. et al. Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation. Nat Med 9, 796–800 (2003). https://doi.org/10.1038/nm879
