Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1
Author: ["Eric Yung","Masha Sorin","Achintya Pal","Errol Craig","Alexei Morozov","Olivier Delattre","John Kappes","David Ott","Ganjam V. Kalpana"]
Publication: Nature Medicine
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Abstract
Integase interactor 1 (INI1), also known as hSNF5, is a protein that interacts with HIV-1 integrase. We report here that a cytoplasmically localized fragment of INI1 (S6; aa183–294) containing the minimal integrase-interaction domain potently inhibits HIV-1 particle production and replication. Mutations in S6 or integrase that disrupt integrase–INI1 interaction abrogated the inhibitory effect. An integrase-deficient HIV-1 transcomplemented with integrase fused to Vpr was not affected by S6. INI1 was specifically incorporated into virions and was required for efficient HIV-1 particle production. These results indicate that INI1 is required for late events in the viral life cycle, and that ectopic expression of S6 inhibits HIV-1 replication in a transdominant manner via its specific interaction with integrase within the context of Gag–Pol, providing a novel strategy to control HIV-1 replication.
Cite this article
Yung, E., Sorin, M., Pal, A. et al. Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1. Nat Med 7, 920–926 (2001). https://doi.org/10.1038/90959
