G-CSF induces E-selectin ligand expression on human myeloid cells

Abstract

Clinical use of G-CSF can result in vascular and inflammatory complications1,2,3,4,5,6,7. To investigate the molecular basis of these effects, we analyzed the adherence of G-CSF–mobilized human peripheral blood leukocytes (ML) to inflamed (TNF-α–stimulated) vascular endothelium. Studies using parallel plate assays under physiologic flow conditions and intravital microscopy in a mouse inflammation model each showed that ML take part in heightened adhesive interactions with endothelium compared to unmobilized (native) blood leukocytes, mediated by markedly increased E-selectin receptor-ligand interactions. Biochemical studies showed that ML express the potent E-selectin ligand HCELL (ref. 8) and another, previously unrecognized ∼65-kDa E-selectin ligand, and possess enhanced levels of transcripts encoding glycosyltransferases (ST3GalIV, FucT-IV and FucT-VII) conferring glycan modifications associated with E-selectin ligand activity. Enzymatic treatments and physiologic binding assays showed that HCELL and the ∼65-kDa E-selectin ligand contribute prominently to the observed G-CSF–induced myeloid cell adhesion to inflamed endothelium. Treatment of normal human bone marrow cells with a pharmacokinetically relevant concentration of G-CSF in vitro9,10 resulted in increased expression of these two molecules, coincident with increased transcripts encoding pertinent glycosyltransferases and heightened E-selectin binding. These findings provide direct evidence for a role of G-CSF in the induction of E-selectin ligands on myeloid cells, thus providing mechanistic insight into the pathobiology of G-CSF complications.

Cite this article

Dagia, N., Gadhoum, S., Knoblauch, C. et al. G-CSF induces E-selectin ligand expression on human myeloid cells. Nat Med 12, 1185–1190 (2006). https://doi.org/10.1038/nm1470

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