Author: ["Mireille Toebes","Miriam Coccoris","Adriaan Bins","Boris Rodenko","Raquel Gomez","Nella J Nieuwkoop","Willeke van de Kasteele","Guus F Rimmelzwaan","John B A G Haanen","Huib Ovaa","Ton N M Schumacher"]
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Abstract
Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome.Cite this article
Toebes, M., Coccoris, M., Bins, A. et al. Design and use of conditional MHC class I ligands. Nat Med 12, 246–251 (2006). https://doi.org/10.1038/nm1360 